Table 2 Genomic location on chromosome 6 and amplicon primer sequences for ESR1 mutation profiling with NGS
From: Estrogen receptor (ESR1) mutation in bone metastases from breast cancer
Start | End | Amplicon length | Forward primer | Reverse primer | |
|---|---|---|---|---|---|
Exon 4 | 152265409 | 152265540 | 131 bp | 5′-GGTCTGCTGGAGACATGAGAG-3′ | 5′-TCACTGAAGGGTCTGGTAGGATC-3′ |
Exon 7 | 152415468 | 152415596 | 128 bp | 5′-CCATGAACACTCTGGGTCTCCTA-3′ | 5′-CATCAGGTGGATCAAAGTGTCTGT-3′ |
Exon 8 | 152419900 | 152420028 | 128 bp | 5′-TGGAGCATCTGTACAGCATGAAG-3′ | 5′-TGCAAGGAATGCGATGAAGTAGAG-3′ |
