Table 3 Pyrosequencing primer used for mutation profiling in exon 5 and for validation of mutations in exon 4, 7, and 8
From: Estrogen receptor (ESR1) mutation in bone metastases from breast cancer
ESR1 exon 4 for | 5′-GGAGACATGAGAGCTGCCAACC-3′ |
ESR1 exon 4 rev | 5′-AATAGAGTATCGGGGGCTCAGCA-3′ |
ESR1 exon 4 pyroseq for | 5′-ATGATCAAACGCTCTAAG-3′ |
ESR1 exon 4 pyroseq rev | 5′-AAGGCCAGGCTGTTC-3′ |
ESR1 exon 5 for | 5′-GTTTTCAGGCTTTGTGGATTTGAC-3′ |
ESR1 exon 5 rev | 5′-ACGAGACCAATCATCAGGATCTCT-3′ |
ESR1 exon 5 pyroseq for | 5′-TCTAGCCAGGCACATT-3′ |
ESR1 exon 5 pyroseq rev | 5′-ATCAGGTCCACCTTCTA-3′ |
ESR1 exon 7 for | 5′-TCTCTGCGCATTCAGGAGTGT-3′ |
ESR1 exon 7 rev | 5′-TGCCTTGGCCATCAGGTG-3′ |
ESR1 exon 7 pyroseq for | 5′-GGAGTGTACACATTTCTG-3′ |
ESR1 exon 7 pyroseq rev | 5′-AGAGACTTCAGGGTGCTGG-3′ |
ESR1 exon 8 for | 5′-TGGAGCATCTGTACAGCATGAAG-3′ |
ESR1 exon 8 rev | 5′-GGCGTCCAGCATCTCCAG-3′ |
ESR1 exon 8 pyroseq for | 5′-AAGAACGTGGTGCCCC-3′ |
ESR1 exon 8 pyroseq rev | 5′-GCATCTCCAGCAGCAG-3′ |
