Table 1 Oligonucleotide sequences

From: Schizophrenia is associated with an increase in cortical microRNA biogenesis

Type	Name	Sequence	Target
Primers^a	U6-probe	5′-GCCATGCTAATCTTCTCTGTATC-3′	U6 snRNA
	U6-F339	5′-CGGCAGCACATATACTAAAATTGG-3′	U6 snRNA
	U49-F	5′-ATCACTAATAGGAAGTGCCGTC-3′	U49 snoRNA
	U49-R	5′-ACAGGAGTAGTCTTCGTCAGT-3′	U49 snoRNA
	U44-F	5′-TGATAGCAAATGCTGACTGA-3′	U44 snoRNA
	U44-R	5′-CAGTTAGAGCTAATTAAGACC-3′T	U44 snoRNA
	107-F	5′-AGCAGCATTGTACAG-3′	miR-107
	107-R	5′-GTAAAACGACGGCCAGTTGATAGCC-3′	miR-107
	15a-F^b	5′-T+AG+CAGCACATAA-3′	miR-15a
	15a-R	5′-GTAAAACGACGGCCAGTCACAAACCA-3′	miR-15a
	15b-F	5′-TAGCAGCACATCAT-3′	miR-15b
	15b-R	5′-GTAAAACGACGGCCAGTTGTAAACC-3′	miR-15b
	16-F	5′-TAGCAGCACATCAT-3′	miR-16
	16-R	5′-GTAAAACGACGGCCAGTTGTAAACC-3′	miR-16
	128a-F	5′-TCACAGTGAACCG-3′	miR-128a
	128a-R	5′-GTAAAACGACGGCCAGTAAAAGAGAC-3′	miR-128a
	181a-F	5′-AACATTCAACGCTG-3′	miR-181a
	181a-R	5′-GTAAAACGACGGCCAGTACTCACCGA-3′	miR-181a
	181b-F	5′-TTTCTAACATTCATTGCT-3′	miR-181b
	181b-R	5′-CAACCTTCTCCCACCGAC-3′	miR-181b
	195-F	5′-T+AGCAGCACAGA-3′	miR-195
	195-R	5′-GTAAAACGACGGCCAGTGCCAATATT-3′	miR-195
	19a-F	5′-TGTGCAAATCTATGC-3′	miR-19a
	19a-R	5′-GTAAAACGACGGCCAGTTCAGTTTT-3′	miR-19a
	20a-F	5′-T+AA+AGTGCTTATAGTG-3′	miR-20a
	20a-R	5′-GTAAAACGACGGCCAGTCTACCTG-3′	miR-20a
	219-F	5′-T+GAT+TGTCCAAAC-3′	miR-219
	219-R	5′-GTAAAACGACGGCCAGTAGAATTGC-3′	miR-219
	26b-F	5′-TT+CA+AGTAATTCAGG-3′	miR-26b
	26b-R	5′-GTAAAACGACGGCCAGTAACCTAT-3′	miR-26b
	27a-F	5′-TT+CACAGTGGCTA-3′	miR-27a
	27a-R	5′-GTAAAACGACGGCCAGTGCGGAACT-3′	miR-27a
	29b-F	5′-T+AG+CACCATTTGAA-3′	miR-29c
	29c-R	5′-GTAAAACGACGGCCAGTTAACCGAT-3′	miR-29c
	338-F	5′-AA+CAATATCCTGGT-3′	miR-338
	338-R	5′-GTAAAACGACGGCCAGTCACTCAGC-3′	miR-338
	7-F	5′-T+GGAAGACTAGTGA-3′	miR-7
	7-R	5′-GTAAAACGACGGCCAGTACAACAAAA-3′	miR-7
	let-7d-F	5′-AGA+GGTAGTAGGTT-3′	let-7d
	let-7d-R	5′-GTAAAACGACGGCCAGTAACTATGC-3′	let-7d
	let-7e-F	5′-TG+AGGTAGGAGGT-3′	let-7e
	let-7e-R	5′-GTAAAACGACGGCCAGTACTATACA-3′	let-7e
	M13-F	5′-GTAAAACGACGGCCAGT-3′	Rev primer for miRNA Q-PCR
	GUSB-F	5′-GCCAATGAAACCAGGTATCCC-3′	GUSB
	GUSB-R	5′-GCTCAAGTAAACAGGCTGTTTTCC-3′	GUSB
	HMBS-F	5′-GAGAGTGATTCGCGTGGGTA-3′	HMBS
	HMBS-R	5′-CAGGGTACGAGGCTTTCAAT-3′	HMBS
	FXR2-F	5′-ACCGCCAGCCAGTGACTGTG-3′	FXR2
	FXR2-R	5′-AGTCACCCTTCTGTCCTGAAA-3′	FXR2
	DICER1-F	5′-CACATCAATAGATACTGTGCT-3′	DICER
	DICER-R	5′-TTGGTGGACCAACAATGGAGG-3′	DICER
	DGCR8-F	5′-GCTGAGGAAAGGGAGGAG-3′	DGCR8
	DGCR8-R	5′-ACGTCCACGGTGCACAG-3′	DGCR8
	DROSHA-F	5′-AAGCGTTAATAGGAGCTGTTTACT-3′	DROSHA
	DROSHA-R	5′-CGTCCAAATAACTGCTTGGCT-3′	DROSHA
	XPO5-F	5′-ATATATGAGGCACTGCGCC-3′	EXP-5
	XPO5-R	5′-AAACTGGTCCAGTGAGTCCTT-3′	EXP-5
	DDX26-F2	5′-AGATCCGAAAGCCAGGAAGAAAA-3′	DDX26
	DDX26-R2	5′-TTTGTAAACTGCCTTGCACATGC-3′	DDX26
	DDX5-F	5′-AAGGATGAAAAACTTATTCGT-3′	DDX5
	DDX5-R	5′-TTTTCCATGTTTGAATTCATT-3′	DDX5
	DDX17-F	5′-GTGAAAAAGACCACAAGTTGA-3′	DDX17
	DDX17-R	5′-TACACATAGCTGGCCAACCAT-3′	DDX17
	FXR2-F	5′-ACCGCCAGCCAGTGACTGTG-3′	FXR2
	FXR2-R	5′-AGTCACCCTTCTGTCCTGAAA-3′	FXR2
	pri-181b-2-F1	5′-AAGAAGAGCCAGGAGTCAGC-3′	pri-181b-2
	pri-181b-2-R1	5′-TCAGTTGGTGGGGTTGCCTT-3′	pri-181b-2
	pre-181b-2-F	5′-CTGATGGCTGCACTCAACAT-3′	pre-181b
	pre-181b-2-R	5′-TGATCAGTGAGTTGATTCAGACT-3′	pre-181b
	pri-26b-F	5′-CCGTGCTGTGCTCCCT-3′	pri-26b
	pri-26b-R	5′-CGAGCCAAGTAATGGAGAACAG-3′	pri-26b
	pre-26b-F	5′-GACCCAGTTCAAGTAATTCAGGA-3′	pre-26b
	pre-26b-R	5′-CGAGCCAAGTAATGGAGAACAG-3′	pre-26b
Cassettes^c	VSNL1-107-T	5′-CTAGTTCCTCCAAAGCCTGGGCAGAAATGTGCTGCAAA-3′	VSNL1
	VSNL1-107-B	5′-AGCTTTTGCAGCACATTTCTGCCCAGGCTTTGGAGGAA-3′	VSNL1
	RELN-107-T	5′-CTAGTTTACTTGTTATGTTGTAATATTTTGCTGCTGAATTT-3′	RELN
	RELN-107-B	5′-AGCTAAATTCAGCAGCAAAATATTACAACATAACAAGTAAA-3′	RELN
	HTR2A-107-T	5′-CTAGCTATTTTCAAGTGGAAACCTTGCTGCTATGCTGTTCA-3′	HTR2A
	HTR2A-107-B	5′-AGCTTGAACAGCATAGCAGCAAGGTTTCCACTTGAAAATAG-3′	HTR2A
	GRIN3A-107-T	5′-CTAGGCACAAACCCTATCAAGAGCTGCTGCTTCCCT-3′	GRIN3A
	GRIN3A-107-B	5′-AGCTAGGGAAGCAGCAGCTCTTGATAGGGTTTGTGC-3′	GRIN3A
	PLEXNA2-107-T	5′-CTAGGACAGTTCTGCCTCTGTGACTGCTGCTTTGCATG-3′	PLEXNA2
	PLEXNA2-107-B	5′-AGCTCATGCAAAGCAGCAGTCACAGAGGCAGAACTGTC-3′	PLEXNA2
	DLG4-107-T	5′-CTAGGTCCGGGAGCCAGGGAAGACTGGAAATGCTGCCG-3′	DLG4
	DLG4-107-B	5′-AGCTCGGCAGCATTTCCAGTCTTCCCTGGCTCCCGGAC-3′	DLG4
	DRD1-107-T	5′-CTAGAATTTACGATCTTAGGTGGTAATGAAAAGTATATGCTGCTTTG-3′	DRD1
	DRD1-107-B	5′-AGCTCAAAGCAGCATATACTTTTCATTACCACCTAAGATCGTAAATT-3′	DRD1
	GRM7-107-T	5′-CTAGGTTTGTAATAAGTACTTTCGTTAATCTTGCTGCTTATGTG-3′	GRM7
	GRM7-107-B	5′-AGCTCACATAAGCAGCAAGATTAACGAAAGTACTTATTACAAAC-3′	GRM7
	RGS4-107-T	5′-AATGCACTAGTCCACATTGTAGCCTAATATTCATGCTGCCTGCCA TGAAGCTTAATGC-3′	RGS4
	RGS4-107-B	5′GCATTAAGCTTCATGGCAGGCAGCATGAATATTAGGCTACAATGTGGA CTAGTGCATT-3′	RGS4
miRNA^d	miR-107+	5′-AGCAGCAUUGUACAGGGCUAUCA-3′	miR-107
	miR-107−	5′-AUAGCCCUGUACAAUGCUGUAUU-3′	miR-107
	miR-15a+	5′-UAGCAGCACAUAAUGGUUUGUG-3′	miR-15a
	miR-15a−	5′-CAAACCAUUAUGUGCUGUUAUU-3′	miR-15a
	miR-15b+	5′-UAGCAGCACAUCAUGGUUUACA-3′	miR-15b
	miR-15b−	5′-UAAACCAUGAUGUGCUGUUAUU-3′	miR-15b
	miR-16+	5′-UAGCAGCACGUAAAUAUUGGCG-3′	miR-16
	miR-16−	5′-CCAAUAUUUACGUGCUGUUAUU-3′	miR-16
	miR-195+	5′-UAGCAGCACAGAAAUAUUGGC-3′	miR-195
	miR-195−	5′-CAAUAUUUCUGUGCUGUUAUU-3′	miR-195
	control-miRNA-1+	5′-AUCCACCACGUAAAUAUUGGCG-3′	miR-15 family
	control-miRNA-1−	5′-CCAAUAUUUACGUGGUGGAUCG-3′	miR-15 family
	control-miRNA-2+	5′-UCCACCAAUGUACAGGGCUAUCA-3′	miR-107
	control-miRNA-2−	5′-AUAGCCCUGUACAUUGGUGAAUU-3′	miR-107
Anti-miRs^e	Anti-miR-107	5′-T+GAT+AGC+CCT+GTA+CAA+TGC+TG-3′	miR-107
	Anti-miR-15a	5′-C+ACA+AAC+CAT+TAT+GTG+CTG+CTA-3′	miR-15a
	Anti-miR-15b	5′-T+GTA+AAC+CAT+GAT+GTG+CTG+CTA-3′	miR-15b
	Anti-miR-16	5′-C+GCC+AAT+ATT+TAC+GTG+CTG+CTA-3′	miR-16
	Anti-miR-195	5′-G+CCA+ATA+TTT+CTG+TGC+TGC+TA-3′	miR-195
	Control anti-miR-1	5′-C+GCC+AAT+ATT+TAC+GTG+GTG+GAT-3′	miR-15 family
	Control anti-miR-2	5′-T+GAT+AGC+CCT+GTA+CAT+TGG+TG-3′	miR-107

^aThe direction of primers with respect to the target sequence was denoted as either F or R for forward and reverse, respectively. The underlined sequence is not gene specific and was used to provide a primer recognition sequence. For miRNA Q-PCR, the Rev primer is used for reverse transcription, and the For primer is used in the Q-PCR with M13-F as the reverse primer.
^bThe positions of LNA-modified bases are preceded by a ‘+’ symbol.
^cSpeI/HindIII cassettes containing putative target recognition elements were used to generate recombinant luciferase reporter gene constructs. ‘T’ indicates top strand and ‘B’ indicates bottom strand.
^dSynthetic miRNAs are used to over-express microRNA. ‘+’ indicates top strand and ‘–‘ indicates bottom strand.
^eAnti-miRs are used to suppress endogenous microRNA.

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Table 1 Oligonucleotide sequences

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