Figure 3
From: Antidepressants increase human hippocampal neurogenesis by activating the glucocorticoid receptor
The effects of sertraline on neuronal differentiation are mediated by phosphodiesterase type 4 (PDE4)/protein kinase A (PKA) signaling. Rolipram (100 nM) increased the effect of SERT (1 μM) on the number of microtubulin-associated protein-2 (MAP2)-positive neurons, whereas H89 abolished it. Rolipram and H89 also counteracted the effect of DEX (1 μM) on MAP2-positive neurons (a). Rolipram increased the effect of SERT on the number of doublecortin (Dcx)-positive neuroblasts, whereas H89 abolished it. Rolipram also counteracted the effect of DEX (1 μM) on Dcx-positive neuroblasts (b). Five independent experiments were conducted on five independent cultures (n=5), four wells were analyzed per treatment condition in each experiment and three random, non-overlapping pictures were analyzed for each well. All data are mean±s.e.m. *P<0.05, **P<0.01 compared with SERT or DEX alone.
