Figure 4
From: Antidepressants increase human hippocampal neurogenesis by activating the glucocorticoid receptor
Effects of sertraline on progenitor cell proliferation. SERT (1 μM) and DEX (1 μM) decreased the number of 5′-bromodeoxyuridine (BrdU)-positive cells. These effects were abolished by the glucocorticoid receptor (GR)-antagonist RU486 (50 nM). Co-treatment of DEX and SERT increased the number of BrdU-positive cells. This effect was also abolished by RU486 (*P<0.05, **P<0.01) (a). Rolipram (100 nM) enhanced the effect of SERT (b, white columns), and of SERT and DEX co-treatment (b, squared columns), whereas H89 (50 nM) abolished both effects. The number of BrdU-positive cells was significantly increased when cells were co-treated with rolipram and DEX (b, striped columns). Six independent experiments were conducted on six independent cultures (n=6), four wells were analyzed per treatment condition in each experiment and three random, non-overlapping pictures were analyzed for each well. All data are mean±s.e.m. *P<0.05, **P<0.01 and ***P<0.001 compared with the respective vehicle or as indicated.
