Figure 4
The Shank3 mutations decreased spontaneous neuronal activity. (a) Miniature EPSCs (mEPSCs) recorded at −60 mV on hippocampal neurons (DIV21) transiently co-transfected with green fluorescent protein (GFP)–C3, GFP–Shank3WT or mutated forms. (b) Quantification of mEPSCs frequency and (c) amplitude under different transfection conditions. In Shank3WT, there is a significant increase in mEPSC frequency. Each bar of the histogram is the mean±s.e.m. of 10 experiments. ***P<0.001 compared with control; °°°P<0.001 and °P<0.05 compared with Shank3WT.
