Figure 2
From: Bidirectional regulation of emotional memory by 5-HT1B receptors involves hippocampal p11
Reversal of enhanced memory responses to 5-HT1BR stimulation in p11KO mice by hippocampal AAV mediated p11 gene transfer. YFP (a–d) and p11 (e–h) in situ hybridization confirmed predominantly hippocampal transduction of the viral vector in mice receiving AAV.YFP (a–b) and expression of p11 mRNA in mice receiving AAV.p11 (e–f). Autoradiograms from coronal brain sections at the level of raphe nuclei indicate no signs of retrograde transport of AAV in hippocampal 5-HT afferents back to the raphe nuclei (c–d, g–h). Schematic illustration of brain region targeted with AAV (I) as defined and adopted from the mouse brain atlas.53 Restoration of 5-HT1BR–like binding by [125I]cyanopindolol autoradiography in p11KO mice after intrahippocampal injection of AAV.p11 (j). Training (k) and emotional memory (l) performance in control groups of mice given AAV.YFP. CP94253 induced opposite effects on training in WT and P11KO mice (k). Basal impairments of memory performance in vehicle P11KO group and biphasic effect of CP94253 in WT and P11KO (l). The abnormal pharmacological response to CP94253 was normalized in p11KO mice receiving hippocampal AAV.p11 at training (m) and testing (n). Learning-related avoidance was induced in the WT vehicle and P11KO CP94253 groups treated with AAV.YFP control vector (l) and P11KO vehicle treated with AAV.p11 overexpressing vector (n). Data are presented as means±s.e.m. n=6–10 mice per group. +++P<0.001 indicates significant difference between training and testing performances. CP: CP94253 (5-HT1BR agonist), P11KO: p11 knock-out mice, WT: wild type mice, Veh: vehicle injection, G: genotype, AAV: adeno-associated viral vector. *P<0.05; **P<0.01; ***P<0.001; #P<0.05; ##P<0.01.
