Figure 5
From: Clusterin regulates β-amyloid toxicity via Dickkopf-1-driven induction of the wnt–PCP–JNK pathway
Transgenic overexpression of Dickkopf-1 (DKK1) induces ‘Aβ neurotoxicity pathway genes’, tau phosphorylation and cognitive deficits. (a) DKK1 expression was determined by qRT-PCR (left bar graph) and enzyme-linked immunosorbent assay (ELISA; (right bar graph) in temporal cortex of neonatal DKK1 transgenic (TG) mice (n=6) and their non-transgenic (non-TG) littermates (n=5). (b) Neonatal expression of the five Aβ/Dkk1 genes determined by qRT-PCR. (c) Immunoblots of hippocampal lysates from 18- to 24-month-old DKK1 TG (n=7) and non-TG (n=7) mice using antibodies AT8 and PHF-1. Phosphoimmunoreactivity values were normalised to total tau values, within blot (bar charts, right). (d) The 14–16-month-old Dkk1 TG (n=17) and non-TG littermates (n=16) were subjected to contextual fear conditioning. Time spent freezing upon placement in the conditioning apparatus at baseline and at 24 h after training are shown. qRT-PCR, quantitative reverse transcription polymerase chain reaction.
