Figure 2
From: Identification and functional characterization of rare SHANK2 variants in schizophrenia
Functional analysis of SHANK2 mutations in primary hippocampal neurons. (a) SHANK2 wild type and mutant (S610Y, R958S, P1119T, A1731S) constructs as N-terminal mCherry fusions. Western blot analysis in HEK293 cells displaying the sizes of the different constructs. (b) Representative hippocampal neurons transfected with GFP and mCherry-SHANK2-wild type (WT) or mCherry-mutants. (c) Quantification of the relative dendritic spine volume and the spine density in primary hippocampal neurons overexpressing the different SHANK2 constructs. (d) Rescue of the Shank2 knockdown. Human SHANK2 wild-type protein normalizes spine volume in Shank2 knockdown neurons, whereas the SHANK2-S231Y and the P740T mutant have lost this ability. No effect was seen on spine density. All values are shown as mean±standard deviation. The comparisons of mean differences between groups were made using two-way analysis of variance test including the different conditions and controlling for the replicates followed by Scheffé post hoc test. **P⩽0.01, ***P⩽0.001; (c and d; n=3 experiments, 18 neurons for each condition).
