Figure 4
(a) Plaque load (%) in both TG groups assessed by methoxy-X04 staining. Each dot represents the histochemically determined plaque load, using Aβ-PET baseline estimate as covariate. Red indicates TG-GSM animals and green shows TG-VEH animals. (b) Plaque density using Aβ-PET baseline estimate as covariate for one hemisphere of each TG mouse. Red indicates TG-GSM animals and green shows TG-VEH animals. The horizontal line in the middle represents the mean value. *indicates statistically significant at P<0.05. (c) Histogram plotting of plaque size revealed a differing distribution between groups of TG mice, with significantly fewer small plaques in the TG-GSM animals (red). (d) Methoxy-X04 staining of representative sagittal slices in the three above mentioned animals, the right panel zooms into the areas with the largest clusters of amyloid plaques in frontal parts of cerebral cortex. (e) Correlation of final Aβ-PET estimates and plaque density shows excellent agreement. Corresponding hemispheres were used from TG-GSM (red squares) and TG-VEH (green circles) animals for this comparison. (f) Correlation of final Aβ-PET estimates and insoluble Aβ42 levels shows excellent agreement. Corresponding hemispheres were used from TG-GSM (red squares) and TG-VEH (green circles) animals for this comparison. (g) Correlation of Aβ42 levels and plaque density shows excellent agreement. Contralateral hemispheres were used from TG-GSM (red squares) and TG-VEH (green circles) animals for this comparison.
