Figure 1
From: Cellular and molecular basis for stress-induced depression
Chronic stress induces loss of p11 in PrL, as well as depression-like behavior. (a) Immunofluorescence image illustrating p11-positive cells (EGFP+) in mPFC. Scale bar, 500 μm. (b) High-magnification image of p11 expression in different layers of PrL and IL. Scale bar, 100 μm. (c) p11-expressing cells in the PrL and IL from control (CON) and chronic restraint stressed (RST) mice. Scale bar, 40 μm. (d) High-magnification images of p11 expression in PrL layer II/III and IL layer V from control and stressed mice. Scale bar, 25 μm. (e) Quantification of p11-expressing cells in PrL (layer II/III) and IL (layer V) from control and stressed mice (n=5 per group). (f–i) Chronic stress-induced depression-like behaviors measured by immobility time in TST (f), FST (g), the ratio of sucrose to water consumption in (h) and the latency to feed in NSF test (i) (n=24 per group). *P<0.05, **P<0.01, two-tailed t-test. ACC, anterior cingulate cortex; FST, forced swin test; fmi, forceps minor of the corpus callosum; IL, infralimbic cortex; M2, motor cortex 2; NSF, novelty-supressed feeding; PrL, prelimbic cortex; SPT, sucrose preference test. Data are means±s.e.m.
