Figure 2
C57BL/6J mice compartmentalize PDE11A4 protein in VHIPP differently than do BALB/cJ mice. To determine whether PDE11A4 protein expression differences between C57BL/6J and BALB/cJ mice might be specific to select subcellular compartments, DHIPP and VHIPP samples were biochemically fractionated and then (a) probed by Western blot. (b) C57BL/6J mice showed lower PDE11A4 protein expression relative to BALB/cJ mice in both the cytosolic and membrane compartments of the DHIPP, (c) but only in the membrane compartment of the VHIPP. This effect was replicated in a second cohort of mice (Supplementary Figure S3). DHIPP, n=6 per strain for membrane and 8 per strain for cytosol; VHIPP, n=8 per strain per compartment. Post hoc, *vs BALB/cJ, P<0.01–0.001, #vs cytosol, P<0.001. Data sets passed normality and equal variance and are graphed as means ±s.e.m. Brightness and contrast adjusted for graphical clarity of blot images. DHIPP, dorsal hippocampus; PDE11A, phosphodiesterase 11A; VHIPP, ventral hippocampus.
