Figure 5
Decreasing PDE11A expression increases IL-6 expression. To determine whether decreased PDE11A4 may drive expression of pathophysiological markers, we examined expression of the pro-inflammatory cytokine IL-6. (a) Western blots show that (b) C57BL/6J mice express more IL-6 relative to BALB/cJ mice in both the cytosolic and membrane fractions of DHIPP (n=6 per strain for membrane and 8 per strain for cytosol). (c) In VHIPP, however, C57BL/6J mice only express more IL-6 relative to BALB/cJ mice in the cytosolic fraction (n=8 per strain per compartment). (d) Interestingly, PDE11A4 expression in the VHIPP membrane (VM) fraction correlates negatively with IL-6 expression in the VHIPP cytosol (VC), such that C57BL/6J and BALB/cJ mice with lower PDE11A4 expression exhibit higher levels of IL-6 expression. (e) Decreasing PDE11A expression appears sufficient to upregulate IL-6 because PDE11A KO mice demonstrate significantly more IL-6 expression relative to paired sex-matched WT littermates in DHIPP (WT, n=17; KO, n=19). Data in panel b passed normality and equal variance. Data in panel c and e failed normality; therefore, nonparametric tests were used for those data sets. Data are graphed as means ±s.e.m. Brightness and contrast adjusted for graphical clarity of blot images. Post hoc, *vs BALB/cJ or WT, P≤ 0.005–0.001. DHIPP, dorsal hippocampus; IL-6, interleukin-6; KO, knockout; PDE11A, phosphodiesterase 11A; VHIPP, ventral hippocampus.
