Figure 3
Antidepressant effects of MAGL inhibitors, fluoxetine and ketamine on chronic CORT-treated mice (a, d) Chronic CORT-treated mice show increased immobility (a) and decreased sucrose consumption (d). (b, c, e, f) An intraperitoneal (i.p.) injection of 20 but not 5 mg kg−1 JZL184 decreases immobility (b) and increases sucrose intake (e), as the decreased immobility is blocked by muscimol pretreatment (c) and lasts for 1 week (f). (g) JZL184 (20 mg kg−1, i.p., 3 × 1/5 days) decreases immobility. (h) An i.p. injection of 40 but not 10 mg kg−1 KML29 decreases immobility. (i) Chronic but not a single or sub-chronic injection of fluoxetine (10 mg kg−1, i.p.) decreases immobility. (j, k) An i.p. injection of 10 mg kg−1 ketamine decreases immobility (j) without significant effects on sucrose intake (k) in mice in response to acute stress, whereas JZL184 (20 mg kg−1, i.p.) increases sucrose intake in mice in response to acute stress (k). All summary graphs show means±s.e.m.s; n=numbers of animals recorded in each group. *P<0.05 and **P<0.01 vs control, #P<0.01 vs vehicle, t test (a, d) or LSD post-hoc test after one-way ANOVA (b: F3,36=27.001, P<0.01; c: F2,27=7.426, P<0.01; e: F3,42=24.974, P<0.001; f: F2,33=26.956, P<0.01; g: F2,27=12.301, P<0.05; h: F3,36=21.890, P<0.01; i: F3,36=39.231, P<0.05; j: F2,27=12.505, P<0.001; k: F4,70=41.114, P<0.01).
