Figure 4
Dyrk1a (dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 1 A) inhibited total neurite outgrowth in rat hippocampal neurons, and short hairpin RNA (shRNA)-mediated loss of function was rescued by wild-type Dyrk1a. (a, c) The morphology of neurons in each condition expressing the indicated plasmids. (a) Neurons expressed CAG vector, Dyrk1a short hairpin RNA (shRNA), Dyrk1a wild-type (WT) and R205X. (c) Neurons expressed specific plasmids for the rescue conditions, including WT and the five mutant forms of Dyrk1a plus Dyrk1a shRNA. (b, d) Statistical analysis of total neurite length in overexpression (b) and rescue conditions (d). Scale bar 50 μm. Approximately 50 neurons from three independent experiments were counted, and the total neurite length of each cell was determined. Unpaired t-tests were performed for the statistical analysis. **P<0.01, ***P<0.001, ****P<0.0001.
