Figure 6
The two Dyrk1a (dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 1 A) truncations did not disturb Dyrk1a subcellular localization. (a–e) Neurons expressing Dyrk1a wild-type (WT) were labeled with 4,6-diamidino-2-phenylindole (DAPI; to identify nuclei), a Myc epitope tag (to identify the precise location of the expressed protein) and green fluorescent protein (GFP; to identify overall neuronal morphology). Merge 1 shows DAPI and Myc co-labeling, and merge 2 shows DAPI, Myc and GFP labeling. Panels (f–j) and (k–o) depict the two nonsense mutants, respectively. Both R205X and E239X, as well as Dyrk1a WT, localized to the nucleus. The images were merged using the ImageJ software. Scale bar 20 μm.
