Figure 2
Normal mitochondrial structure but abnormal presynaptic cytosolic and mitochondrial calcium regulation in Df(16)5+/− and Mrpl40+/− mice. (a) Three representative transmission electron microscopy images of mitochondrial ultrastructure in the CA1 area of the hippocampus of wild-type (WT) and Df(16)5+/− mice. (b) The representative fluorescent image of mCherry after infection of the CA3 area with adeno-associated viruses (AAVs) encoding either GCaMP6 or mitoGCaMP6. (c) Line scan of mCherry and GCaMP6 fluorescence in a CA3 presynaptic terminal before and after the 80-Hz stimulation of Schaffer collaterals (arrow). (d–g) Mean normalized cytosolic GCaMP6 (d, f) and mitoGCaMP6 (e, g) fluorescence in CA3 presynaptic terminals imaged in the CA1 area of the hippocampus, before and after 80-Hz stimulation in Df(16)5+/− and WT littermates (d, e) and Mrpl40+/− and WT littermates (f, g). (h, i) Normalized mean peak amplitudes of GCaMP6 (h) or mitoGCaMP6 (i) in Df(16)5+/− and WT littermates and Mrpl40+/− and WT littermates. Numbers of fluorescent puncta are shown inside columns. *P<0.05.
