Figure 4 | Molecular Psychiatry

Figure 4

From: Hippocampus-dependent memory and allele-specific gene expression in adult offspring of alcohol-consuming dams after neonatal treatment with thyroxin or metformin

Figure 4

Changes in allele-specific and total expression of hippocampal Dio3 and Igf2 are sex- and treatment-dependent. Allele-specific expression in adult male hippocampus was determined by pyrosequencing using the already-verified single-nucleotide polymorphisms (a) Dio3 (N=4–10 per prenatal diet/neonatal treatment) and (b) Igf2 (N=3–11 per prenatal diet/neonatal treatment). Schematic representation of the maternal and the paternal allelic contributions to the total hippocampal expression of (c) Dio3 and (d) Igf2 (drawn not to the scale). (e) Effects of neonatal metformin treatment on fetal alcohol exposure-altered transcript levels of hippocampal Dio3 in adult offspring (N=4–8 per sex/prenatal diet/neonatal treatment). (f) Total transcript levels of Igf2 in the hippocampus of adult offspring in response to FAE and neonatal T4 treatment (N=8–20 per prenatal diet/neonatal treatment, sex combined). Details are as described in Figure 1. *P<0.05, **P<0.01, Bonferroni post hoc test within prenatal treatments.

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