Figure 3
From: FBXW7 regulates DISC1 stability via the ubiquitin-proteosome system
Substitution analysis pinpoints novel binding motifs for FBXW7. (a) Peptide array grids containing substitution of residues within the core binding motif domain 197PTPPGSH202 (with a range of native and non-native residues) were overlaid with a purified protein complex containing 6His-FBXW7 and Skp1. mD and mE are N-methyl aspartic acid and N-methyl glutamic acids respectively. (d) and (e) are the D-forms of aspartic acid and glutamic acid. (b) Arrays were then probed for 6His and spots that were over 200% of the control identified by densitometry.
