Figure 6
From: FBXW7 regulates DISC1 stability via the ubiquitin-proteosome system
DISC1-FBXW7 disruptor peptides restore DISC1 protein expression in neural progenitors derived from patient iPS cells carrying a DISC1 mutation. (a) Relative DISC1 levels were determined in cellular lysates from neural progenitors derived from different iPS cell lines as described previously.8 (b) Neural progenitors derived from control subjects were treated with the indicated peptides (100 μM) for 3 h prior to cellular lysate being isolated and blotted for DISC1 and GAPDH. Relative DISC1 levels were calculated by densitometry (n=3). (c and d) Neural progenitors derived from patients carrying a 4-bp deletion in the DISC1 gene were treated with the indicated peptides (100 μM) for 3 h prior to cellular lysate being isolated and blotted for DISC1 and GAPDH. Relative DISC1 levels were calculated by densitometry (n=3). (e) Neural progenitors derived from control subjects and patients carrying a 4-bp deletion in the DISC1 gene were treated with the indicated peptides (100 μM) for 3 h prior to cellular lysate being isolated and blotted for DISC1 and GAPDH. Relative DISC1 levels were calculated by densitometry (n=3).
