Figure 5
From: The PNKD gene is associated with Tourette Disorder or Tic disorder in a multiplex family
Endogenous RIMS1α expression in Uaf and TD cells. (a) RIMS1α transcript was not expressed in LCL, was expressed at low levels in iPSC and NPC and was enriched in iPSC-derived D30 neurons (n=3). (b) RT–qPCR measured the RIMS1α transcript in iPSC-derived D30 neurons of Uaf, TD1 and TD2 subjects. D30 neurons from three independent differentiations were collected. There was no significant difference between the expression of the RIMS1α transcript in Uaf and TD neurons (n=3). (c) RIMS1 protein was examined by western blot in D30 neurons from three independent differentiations. RIMS1 protein was reduced in TD neurons. iPSC, iPSC, induced pluripotent stem cell; LCL, lymphoblastoid cell lines; NPC, neural progenitor cell; TD, Tourette disorder.
