Figure 1
Cortical phenotype of Celsr1 mutant mice. (a–c) Pax6 (red) and Gfp (green) staining of coronal brain sections from wild type (a) and Celsr1−/− (b) embryos, electroporated with a Gfp- coding plasmid at e14.5 and collected at e15.5. Scale bars 50 μm. (c) Quantification of Gfp-positive cells residing in the VZ, 24 h after electroporation. n=4 brains for each genotype, error bars are s.e.m., Student’s t-test, P<0.01. (d–f) clonal analysis of aNPC progeny in whole-mounts of the ventricular wall from Celsr1+/+;NestinCreERT2;dTomato (d) and Celsr1f/f;NestinCreERT2;dTomato (e) Scale bars 50 μm. (f) Quantification of the number of cells per clone. 18 % of mutant clones have more than 17 cell per clone (0 % in controls), n=6 whole-mounts for each genotype. (g–l) Immunochemistry for aNPC (Pax6) and BPs (Tbr2) at e12.5 (g, h), e14.5 (i, j) and e16.5 (k, l). Scale bars 50 μm. (m) Quantification of Tbr2-positive cells in lateral pallium at indicated stages. n=3 brains per stage and per genotype, bars represent s.e.m., two-way ANOVA, P<0.001.
