Figure 2
Cortical hypoplasia and abnormal behavior in Celsr1-deficient mice. (a,b) Mature brains from Wild type (a) and Celsr1−/− (b) mice. Note the reduced size in the mutant. (c) Quantification of neurons of different cortical layers. n=3 brains per genotype, bars represent s.e.m., Student’s t-test, P<0.05 for Cux1-positive cells (layers II and III); and P<0.01 for Foxp2-positive cells (layer IV). The number of cells in the Wild type is set to 100%. (d, e) Nissl staining of the adult VZ/SVZ in wild type (d) and Celsr1−/− (e). (f, g) Activity of males in open field. Bars represent s.e.m., Student’s t-test, P<0.01. (h) Time spent by males in open areas of elevated plus maze (EPM). Bars represent s.e.m., Student’s t-test, P<0.05; (i) Learning ability of males in the Water maze. Bars represent s.e.m., Student’s t-test, P<0.05. (j) Social behavior in the ‘3 chamber’ test for males. Bars represent s.e.m., one-way ANOVA, P<0.001 for both genotypes (stranger versus empty chamber). (k, l) Activity of females in open field. Bars represent s.e.m., Student’s t-test, P>0.05. (m) Time spent by females in open areas of elevated plus maze. Bars represent s.e.m., Student’s t-test, P>0.05. (n) Learning ability of females in the Water maze. Bars represent s.e.m., Student’s t-test, P>0.05 (o) Social behavior in the ‘3 chamber’ test for females. Bars represent s.e.m., one-way ANOVA, P<0.001 control females, P>0.05 for mutant females (stranger versus empty chamber). n=14 controls (Celsr1+/+;Emx1-Cre: nine males, five females), n=10 Celsr1Emx1cKO (five males, five females).
