Figure 3
Celsr1 expression during cortical development. (a–i) Coronal sections stained for Celsr1 (green, white), and Phospho-Vimentin (pVimentin, a–c) or Nestin (d–i) (red). At e10.5 (a–c), Celsr1 protein localizes at the apical side (arrowheads) but not in endfeet (brackets). Scale bars 50 μm. At e12.5 (d–f) and e16.5 (g–i), Celsr1 is distributed both in apical and basal compartments. Scale bars 50 μm. (j–o) Coronal sections in forebrain of Wild type (j–l) and Celsr1−/− mice (m–o) immunostained for Nestin (j, m), Gfp (green) and Laminin (red) (k, n), Laminin (green) and Tuj–1 (red) (l, o). Note the intact basal lamina (arrowheads). Scale bars 50 μm (j, m), 10 μm (k, n), 100 μm (l, o). (p, r) Gfp-filled basal processes of aNPC from Wild type (p) and Celsr1−/− mice (q). Note the simplified morphology in the mutant. Scale bars 20 μm. (r) Quantification of endfeet in Wild type and Celsr1−/− mice at P1. n=65 aNPC for Wild type, and 81 for Celsr1−/−; Chi square test, X2=0.002.
