Figure 4
RG endfeet modulate the RA neurogenic signal. (a, b) The RA shuttle protein Crabp2 is enriched in aNPC endfeet. Scale bars 50 μm. (c–f) Modification of transcription of RA target genes Tnc and Igfbp5 revealed by RNA-Seq on isolated aNPC (c, d) and confirmed by qRT-PCR on e14.5 telencephalon (e, f). n=3 brains for RNA-Seq and four brains for qRT-PCR. Bars represent s.e.m. Two-way ANOVA for qRT-PCR. (g–p) RA supplementation from e11.5 to e14.5 rescues the production of BPs (g–k) and lateral expansion of the VZ (l–p). n=4 embryos per condition. Bars represent s.e.m. Two-way ANOVA, P<0.001 without RA and P>0.05 with RA.
