Figure 5
Effect of RA supplementation on cortical development and behavior in Celsr1 Emx1cKO mice. (a) Quantification of Cux1-positive neurons (layers II and III) in Celsr1wildtype, Celsr1Emx1cKO-RA and Celsr1Emx1cKO+RA. n=5 brains per genotype; bars represent s.e.m.; Student’s t-test; Celsr1Wildtype versus Celsr1Emx1cKO-RA, P<0.05; Celsr1Wildtype versus Celsr1Emx1cKO+RA, P>0.05. The number of cells in the Wild type is set to 100%. (b) Locomotor activity of males in open field. Bars represent s.e.m.; Student’s t-test; Celsr1Wildtype versus Celsr1Emx1cKO-RA, P<0.05; Celsr1Wildtype versus Celsr1Emx1cKO+RA, P>0.05. (c, d) Time spent by males in exposed areas of open field (c) and open arms of elevated plus maze (d). Bars represent s.e.m., Student’s t-test, Celsr1Wildtype versus Celsr1Emx1cKO-RA, P<0.05; Celsr1Wildtype versus Celsr1Emx1cKO+RA, P>0.05; (e) Learning ability of males in the water maze. Bars represent s.e.m., Student’s t-test, Celsr1Wildtype versus Celsr1Emx1cKO-RA, P<0.05; Celsr1Wildtype versus Celsr1Emx1cKO+RA, P<0.05. (f) Social behavior in the ‘3 chamber’ test for females. Bars represent s.e.m., one-way ANOVA, P>0.05 for Celsr1Emx1cKO-RA (stranger versus empty chamber); P<0.001 for Celsr1Wildtype and Celsr1Emx1cKO+RA (stranger versus empty chamber). For the RA rescue experiments, n=9 animals per genotype and per condition.
