Figure 2
From: A paternal methyl donor-rich diet altered cognitive and neural functions in offspring mice
Hippocampal Kcnmb2 overexpression improved water maze impairments in MD F1 mice. (a) Experimental design. (b) Representative image of hippocampal slices infected with AAV1-hSyn1-Kcnmb2-IRES-GFP or control AAV1-hSyn1-GFP virus. Green, GFP, imaged 4 weeks after virus injection. Scale bar, 100 μm. (c) Relative Kcnmb2 mRNA expression in the hippocampus after viral infection as determined by qPCR (n=5 mice per group; two-way ANOVA with the between-subjects factors paternal diet and AAV treatment: effect of paternal diet, P=0.0214; effect of AAV treatment, P<0.0001; paternal diet × AAV treatment interaction, P=0.7084). (d and e) Quadrant occupancy measures (d) and target crossings (e) during a probe trial given after completion of water maze training (CD F1 control-AAV, n=12 mice; MD F1 control-AAV, n=12 mice; CD F1 Kcnmb2-AAV, n=8 mice; MD F1 Kcnmb2-AAV, n=8 mice). Pool quadrants: TQ; AR; OQ; and AL. All graphs display mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001. AL, adjacent left; ANOVA, analysis of variance; AR, adjacent right; CD, control diet; GFP, green fluorescent protein; MD, methyl donor-rich diet; OQ, opposite quadrant; TQ, target quadrant.
