Figure 4
EFhd2 controls gene co-expression in the prefrontal cortex (PFC) of mice, a brain region with naturally high EFhd2 expression. (a) Weighted gene co-expression network analysis of mouse PFC expression data comparing alcohol or water drinking EFhd2 knockout (KO) and wild-type (WT) mice (n=4 PER group). Cluster dendrogram generated by hierarchical clustering of genes on the basis of topological overlap. Modules of correlated genes were assigned colours and are indicated by the horizontal bar beneath the dendrogram, where all unassigned genes were placed in the grey module. (b) Expression changes between WT and EFhd2 KO for selected candidate genes (EFhd2, S100a5, Th and Doc2g) of the turquoise model. (c, d) Scatter plot of correlations between gene significance (GS), that is, differential expression between WT and EFhd2 KO (F-statistics from the analysis of variance model) and module membership (MM) for turquoise and green-yellow modules. (e, f) Heatmap of top 100 genes in turquoise and green-yellow modules. In the heatmap, red represents high expression, whereas green represents low expression values.
