Figure 5
EFhd2 controls cortical development and micro-morphology of neuronal cells. (a) In situ hybridization against Efhd2 expression in explanted brains of Xenopus stage 40 tadpoles. A sense probe was used to identify unspecific binding (bar: 0.5 mm). (b) At the eight-cell stage, Xenopus embryos were injected unilaterally into one dorso-animal blastomere with EFhd2 morpholino or an unrelated control morpholino, along with a lacZ-encoding plasmid. The star (*) marks the injected side. At stage 28, β-galactosidase activity was visualized to identify the injected side. Pax6 was detected by whole-mount in situ hybridization (bar: 1 mm). (c) At the eight-cell stage, Xenopus embryos were injected unilaterally into one dorso-animal blastomere with EFhd2 morpholino or an unrelated control morpholino. At stage 28, Eomes expression was assessed by quantitative PCR (***P<0.001). (d) Brain area volume of adult EFhd2 knockout (KO) and wild-type (WT) mice measured with magnetic resonance imaging. Hipp, hippocampus; OB, olfactory bulb; PFC, prefrontal cortex; SMC, sensorimotor cortex; Ventr, ventricle; *P<0.05.
