Figure 6
EFhd2 controls neuronal spine density and dendritic length. (a) Primary cortical neurons were transfected with short hairpin RNAs (shRNAs; scrambled or directed against EFhd2) linked to green fluorescent protein (GFP) expression, with GFP expressing or EFhd2-IRES-GFP vectors, fixed and stained with phalloidine–rhodamine. (b–e) Dendrites and spines of green cells were analysed with ImageJ. Data are shown as mean+s.e.m. (*P<0.05; ***P<0.001).
