Figure 2
From: Uncoupling DAPK1 from NMDA receptor GluN2B subunit exerts rapid antidepressant-like effects
Chronic unpredictable stress increased the expression levels of GluN2B and DAPK1 and their interaction in the mPFC. (a) Timeline of CUS exposure and sample collection (n=8–9 per group). (b) Representative western blots and quantification of fold changes in GluN1, GluN2A, p-GluN2A, GluN2B, p-GluN2B, DAPK1, p-DAPK1, CREB, p-CREB and BDNF in the mPFC relative to control. (c) Co-immunoprecipitation of GluN2B with DAPK1 in the mPFC in control and CUS rats. The quantification analysis revealed a greater interaction between GluN2B and DAPK1 in the mPFC after CUS exposure (n=6 per group). (d) Timeline of surgery, drug microinjection, behavioral testing and sample collection (n=8–9 per group). (e) Microinjection of DHK in the mPFC rapidly decreased sucrose preference but did not affect total fluid consumption. (f) Representative western blots and quantification of fold changes in GluN1, GluN2A, p-GluN2A, GluN2B, p-GluN2B, DAPK1, CREB, p-CREB and BDNF in the mPFC after DHK infusion relative to vehicle. The data are expressed as mean±s.e.m. *P<0.05, **P<0.01, compared with control or vehicle group. BDNF, brain-derived neurotrophic factor; CREB, cyclic AMP response element-binding protein; CUS, chronic unpredictable stress; DAPK1, death-associated protein kinase 1; DHK, dihydrokainate; mPFC, medial prefrontal cortex.
