Figure 3
From: Elevation of p11 in lateral habenula mediates depression-like behavior
Chronic stress induces burst firing of action potentials in lateral habenula (LHb) neurons, which is abolished by knockdown of p11. (a, b) Scatter plots and bar graphs showing the resting membrane potentials (a) and spontaneous action potential (sAP) frequencies (at −45 mV) (b) in control (CON) and stressed (RST) mice with or without lentiviral injection of p11 shRNA (sh-p11) or GFP-shRNA (sh-GFP) (n=12, CON; n=10, RST; n=7, RST+sh-p11; n=6, RST+sh-GFP). (c) Representative sAP traces in the four groups. (d) Representative traces showing the membrane excitability at the hyperpolarized level (−65 mV) in CON and RST mice with or without lentiviral injection of sh-p11 or sh-GFP. Expanded view of the bursting AP trace at the position denoted by *. (e) Bar graphs showing the percent of LHb neurons exhibiting rhythmic bursting APs (at −65 mV) in the four groups (n=13, CON; n=12, RST; n=7, RST+sh-p11; n=6, RST+sh-G).
