Figure 27: Conservation scores for 50-bp windows in a 4.5-kb region containing the human insulin-like growth factor binding protein acid labile subunit (IGFALS) gene.
From: Initial sequencing and comparative analysis of the mouse genome
In the track near the top of figure, the two coding exons of the gene are displayed as taller blue rectangles, UTRs as shorter rectangles, and the intron, which separates the coding exons, is shown as a barbed line indicating direction of transcription (the gene is on the reverse strand). Log probability scores (L-scores) for all 50-bp windows are shown below the gene. The L-score is -log10(p), where p is the probability under the neutral density, Sneutral, of getting a conservation score as high as is observed in the window. Many windows in the coding region get L-scores greater than 3, indicating less than a 1/1,000 chance of occurring under neutral evolution (Pselected(S) > 0.94; see Fig. 28), and some in a local peak in the upstream region of the gene on the right show L-scores greater than 2, indicating less than a 1/100 chance of occurring (Pselected(S) > 0.75). The red bar shows the location of the interferon-γ-activated sequence-like element (GLE), which is bound by transcription factors from the STAT5a and STAT5b protein family to control expression of this gene244,245. Additional regulatory elements may be located in the other peaks of conservation. This figure is taken with permission from the UCSC browser (http://genome.ucsc.edu).
