Figure 1: The compendium of somatic mutations in a small-cell lung cancer genome.
From: A small-cell lung cancer genome with complex signatures of tobacco exposure
a, Power calculations showing the number of true somatic substitutions detected (blue) and mis-calls (single nucleotide polymorphisms (SNPs) called as somatic mutations, burgundy, and sequencing errors called as mutations, green) for different levels of sequence coverage. Calculations are based on a true mutation prevalence of 1 per megabase (black line). b, Histogram of the actual coverage achieved per base of the tumour (blue) and normal (burgundy) genomes. c, Figurative representation of the catalogue of somatic mutations in the genome of NCI-H209. Chromosome ideograms are shown around the outer ring and are oriented pter–qter in a clockwise direction with centromeres indicated in red. Other tracks contain somatic alterations (from outside to inside): validated insertions (light-green rectangles); validated deletions (dark-green rectangles); heterozygous (light-orange bars) and homozygous (dark-orange bars) substitutions shown by density per 10 megabases; coding substitutions (coloured squares; silent in grey, mis-sense in purple, nonsense in red and splice site in black); copy number (blue lines); validated intrachromosomal rearrangements (green lines); and validated interchromosomal rearrangements (purple lines).
