Extended Data Figure 7: Rapamycin induces autophagy in Paneth cells, reduces the severity of enteritis and the number of TUNEL+ intestinal epithelial cells in Xbp1ΔIEC but not in Atg7/Xbp1ΔIEC or Atg16l1/Xbp1ΔIEC mice.
From: Paneth cells as a site of origin for intestinal inflammation
a, b, Quantification of GFP–LC3 punctae accumulation after 3-day tamoxifen treatment and induction by rapamycin (a; n = 10; one-way ANOVA with post-hoc Bonferroni; mean ± s.e.m.) and representative images in b (n = 3). Scale bars, 5 μm. c, Densitometry of the immunoblot in Fig. 4a (n = 3; one-way ANOVA with post-hoc Holm’s-corrected unpaired Student’s t-test; mean ± s.e.m.). d, Quantification of TUNEL+ intestinal epithelial cells per cm of gut in mice treated with rapamycin or vehicle (n = 5; unpaired Student’s t-test; mean ± s.e.m.). e, Representative images of TUNEL-labelled sections from rapamycin- or vehicle-treated mice. Scale bars, 50 μm. f, g, Quantification of TUNEL+ intestinal epithelial cells per cm of gut in mice treated with rapamycin or vehicle (f; n = 4/4/5/5/5/5; g; n = 5; one-way ANOVA with post-hoc Holm’s-corrected unpaired Student’s t-test; mean ± s.e.m.). h, i, Enteritis histology scores of rapamycin- or vehicle-treated mice of respective genotypes (h; n = 8/9/6/9/12/13; i, n = 8/7/6/7/7/6) (median shown; Kruskal–Wallis with post-hoc Holm’s-corrected Mann–Whitney U-test). *P < 0.05, **P < 0.01, ***P < 0.001.
