Extended Data Figure 4: Identifying integration of pHV4 into the main chromosome.
From: Accelerated growth in the absence of DNA replication origins
a, Map of region around ISH18 insertion sequence element HVO_0278 on the main chromosome of wild isolate DS2, showing restriction sites and probes used to determine the integration of pHV4. b, Map illustrating integration of pHV4 into the main chromosome of laboratory strain H26, by recombination between ISH18 HVO_0278 (chromosome) and ISH18 HVO_A0279 (pHV4). Regions upstream and downstream of the integration are depicted with the same restriction sites and probes shown in Extended Data Fig. 4a, in addition to the genomic fragments cloned in pTA1238 and pTA1236. c, Restriction fragment length polymorphisms in the main chromosome of laboratory strain H26. Genomic DNA from wild isolate DS2 and laboratory strain H26 was digested with KpnI, ClaI or NarI, and probed with sequences upstream (US) and downstream (DS) of ISH18 insertion sequence element HVO_0278. The upstream 3,646 bp NarI fragment of H26 was cloned in pTA1238, and the downstream 7,478 bp KpnI fragment of H26 was cloned in pTA1236. See Methods for details.
