Extended Data Figure 6: Comparison between the CSFV and the HCV pseudoknots.
From: Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit

Views of the structures of the HCV pseudoknot, from the 3.6 Å resolution crystal structure, with an additional crystallization module extending from helix III127 (a) (PDB: 3T4B) and the CSFV pseudoknot in the context of the 40S-subunit-bound ΔII-IRES (b) (this study) are shown in ribbon representation and coloured according to the scheme of the respective secondary structure diagrams (Extended Data Fig. 1a). c, d, Close-up views of HCV and CSFV pseudoknots, showing the ‘main’ helix, formed by helix III1 and pseudoknot (pk) stem 1A (in HCV) or helix III1, pk stem 1a and pk stem 1b (in CSFV), and the ‘sidecar’ helix, which contains subdomain IIIe, pk stem 2 and the two-base-pair helical segment of subdomain IIIf (see Extended Data Fig. 5a, b).