Extended Data Figure 8: Formation of elongation-competent 80S ribosomes on the HCV IRES depending on the presence of eIF3.
From: Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit

Toe-printing analysis of 48S initiation and 80S pre-termination complexes (pre-TC) assembled on the wild-type and ΔIIIb HCV (MSTN-STOP) mRNAs with translation components as indicated. The positions of the initiation and stop codons are shown on the left. Lanes C, T, A and G depict the cDNA sequence corresponding to the wild-type HCV (MSTN-STOP) mRNA. The gel reported in the figure is representative of results obtained from three technical replicates.