Extended Data Figure 3: Unsupervised three-dimensional classification of IRES-bound ribosomal complexes.
From: Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit
Unsupervised three-dimensional classification of IRES-bound ribosomal complexes identified ∼423,000 particles inconsistent with the known structure of the 40S subunit (rejects) and six well-populated classes containing complexes of the 40S subunit in a binary complex with the ΔII-IRES (class 1), of the 40S subunit bound to the ΔII-IRES and DHX29 (class 2), of the 40S subunit bound to DHX29 and eIF3 (class 3) and of the 40S subunit bound to the ΔII-IRES, DHX29 and eIF3 in orientation 1 (class 4), in orientation 2 (class 5) and in orientation 3 (class 6), viewed from (left) the back, (centre) the intersubunit side and (right) the solvent side.
