Extended Data Figure 8: Effects of Muc5b expression on pulmonary inflammation.
a, Lung lavage leukocytes from Muc5b+/+ (blue, n = 15, 23, 7), Scgb1a1-Muc5b (green, n = 11, 18, 5), and Muc5b−/− (red, n = 12, 5, 5) mice in the steady state at ages 3, 6 and 12 months and moribund Muc5b−/− animals (red; n = 8, various ages) and Muc5b+/+ cagemates (blue, n = 6). *P < 0.05 by ANOVA (timecourse studies) and by Student’s t-test (moribund Muc5b−/− versus Muc5b+/+ cage mates). b, Three-month-old Muc5b+/+ (blue, n = 7), Scgb1a1-Muc5b (green, n = 3) and Muc5b−/− (red, n = 3) mice were challenged intratracheally with 5 × 107 CFU USA300. Inflammation was assessed 24 h post infection. No significant differences in macrophages or neutrophils were seen among groups. Error bars, s.e.m. c, Young (3 months of age) and ageing (6–7 months of age) Muc5b+/+ (blue), Scgb1a1-Muc5b (green) and Muc5b−/− (red) mice were exposed to 2–5 × 107CFU of live or heat-killed Staphylococcus USA300, or to saline (SAL) as indicated. Lung lavage leukocytes were assessed 7 days post challenge. Ageing Muc5b−/− mice died before lavage sampling. Infected Muc5ac−/− mice showed no differences in inflammation compared to Muc5b+/+ or C57BL/6J (not shown). Error bars, s.e.m. Numbers in brackets, n mice. *P < 0.05 across genotypes by ANOVA and within genotypes by Student’s t-test. Tg, Scgb1a1-Muc5b transgenic.
