Extended Data Figure 1: Generation and characterization of Muc5ac- and Muc5b-mutant mice. | Nature

Extended Data Figure 1: Generation and characterization of Muc5ac- and Muc5b-mutant mice.

From: Muc5b is required for airway defence

Extended Data Figure 1

a, Muc5b-knockout mice were generated by flanking exon 1 with loxP sites, selecting embryonic stem cells for neomycin resistance, and crossing allele-positive mice with germline active FLP and Cre deleter strains. b, Muc5b transgenic mice were generated by inserting a full-length Muc5b genomic DNA (gDNA) transgene driven by the airway specific rat Scgb1a1 promoter. c, Muc5b transgene expression throughout the conducting airways (top part, bronchi; bottom part, bronchioles). Note the lack of Muc5ac induction in Scgb1a1-Muc5b mouse airways (top right). d, Immunoblot analysis of wild-type (WT) and Scgb1a1-Muc5b mouse lung homogenates. e, Pulmonary MCC in Scgb1a1-Muc5b mice. f, Loss of Muc5ac and retention of Muc5b in ovalbumin sensitized and challenged Muc5ac−/− airways. Tg, transgenic.

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