Extended Data Figure 5: Further characterization of bacteria in Muc5b^−/− mouse lungs.

a–e, Bacteria grown from lungs under microaerophilic conditions. a, b, Total growth in 12-week-old Muc5b^−/− and Muc5b^+/+ mice, and in moribund and control mice. c–e, Bacterial genera, staphylococcal species and S. aureus incidence. f–h, Streptococcal group and S. acidominimus growth in mouse lungs. f, Streptococci identified by taxonomic classifications were assessed for abundance (g) and prevalence (h). Symbols identify samples from the lungs of individual Muc5b^+/+ (blue) and Muc5b^−/− (red) mice at 12-week-old (open circles) and moribund or cage-mate control cohorts (filled circles). Data were analysed by t-tests in a, b, ANOVA in c, d, g, and using chi-squared tests in e, h. P < 0.05 was considered significant. Total numbers of mice in each group are listed as denominators below e and h. ND, none detected; NS, not significant.