Extended Data Figure 8: Ultrafast endocytosis is mediated by dynamin.

a–f, Additional representative micrographs showing 80 µM dynasore-treated cells from 40-nm thick sections (a–c) and a 200-nm tomogram (d–f). Ultrafast endocytic structures are trapped on the surface in the dynasore-treated cells. Note that large vesicles are found associated with the membrane (a–c, d, f) but the necks of the trapped structures are only visible at their centre (a, e) due to the thickness of the sections. Electron tomography was performed on 21 sections (200-nm thick). 9 out of 21 tomograms showed a trapped structure, and all of these trapped structures were connected to the membrane at their centre.