Extended Data Figure 4: Cryo-EM of TRPV1 using Tecnai TF20 microscope and TemF816 8k × 8k CMOS camera.
From: Structure of the TRPV1 ion channel determined by electron cryo-microscopy
a–d, Representative images of frozen hydrated TRVP1 in amphipols taken at different defocus levels, 3.1 μm (a) and 1.5 μm (b) and their Fourier transforms (c, d). Thon rings extend to ∼8 Å. Dash-line squares or circles indicate representative particles showing two distinctive views. e, 2D class averages of TRPV1 particles. f, Enlarged view of three representative 2D class averages.
