Extended Data Figure 5: 3D reconstruction of TRPV1 calculated from TF20 data.
From: Structure of the TRPV1 ion channel determined by electron cryo-microscopy
a, Gold-standard FSC curve for the 3D reconstruction, marked with resolutions corresponding to FSC = 0.5 and 0.143. b, Side view of the 3D reconstruction low-pass filtered at 9 Å and amplified by a temperature factor −1,500 Å2, showing transmembrane (top) and cytoplasmic (bottom) domains. The transmembrane domain roughly fitted by the atomic model of NaVAb (PDB 3RVY). c, Longitudinal cross section view focused on central transmembrane helices. d, Bottom-up view of the 3D reconstruction shows overall structure. e, f, Bottom-up cross-section views showing the arrangement of transmembrane (e) and cytoplasmic (f) domains.
