Extended Data Figure 8: 3D reconstruction of TRPV1 calculated from TF30 data.
From: Structure of the TRPV1 ion channel determined by electron cryo-microscopy
a, Gold-standard FSC curve (red) of the final 3D reconstruction, marked with resolutions corresponding to FSC = 0.5 and 0.143. The FSC curve between the final map and that calculated from the atomic model is shown in blue. The relative low value of this FSC (blue) at low frequency range (>10 Å) is probably due to the presence of amphipol density in the experimental map. b, Euler angle distribution of all particles used for calculating the final 3D reconstruction. The sizes of balls represent the number of particles. The accuracy of rotation is 3.54°, as reported by RELION. c, Different views of the 3D reconstruction low-pass filtered at 6 Å and amplified by a temperature factor of −100 Å2, fitted with the atomic model of TRPV1. d, Two views of the 3D reconstruction displayed at two different isosurface levels (high in yellow and low in grey). At the low isosurface level, the belt-shaped density of amphipols is visible with a thickness of ∼30 Å.
