Extended Data Figure 7: Observed densities in vanilloid pocket.
From: TRPV1 structures in distinct conformations reveal activation mechanisms
a, Non-protein associated densities in the region adjacent to S3–S4 transmembrane helices observed in 3D density maps of the apo TRPV1 structure (blue, 3.4 Å, −100 Å2) or TRPV1 in complex with RTX/DkTx (red, 3.8 Å, −150 Å2) or capsaicin (yellow, 3.9 Å, −150 Å2), as indicated. b, Density of bound capsaicin (blue) viewed from the side (left) and top-down (that is, from the extracellular face; right). Density is also observed in the apo-channel structure (purple), possibly representing an endogenous lipid or other small hydrophobic molecule. All maps were low-pass filtered to 4.5 Å with a temperature factor of −200 Å2, normalized and displayed at the same sigma level (8σ).
