Extended Data Figure 2: Enrichment of Sec-Pro by chemical inhibition of notch signalling.
From: Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity
a, Quantitative RT–PCR data showing peak Atoh1 expression ∼38 h after treatment of wild-type mice with dibenzazepine (DBZ). b–d, Low magnification views show no increase in mature, Alcian-blue-avid goblet cells 38 h after DBZ treatment (b), appearance of goblet cells in villus bases and crypts 72 h after DBZ treatment (c), and migration of these cells to occupy villi almost fully within 6 days (d). Inset in b shows a magnified view of the boxed area. e, Within 72 h of DBZ treatment, crypt cells virtually cease to replicate (E), retain ATOH1 (C), and stain with Alcian blue (A), a sign of goblet cell maturation. By 120 h after DBZ exposure, these ATOH1+ post-mitotic goblet cells occupy short villi (B, D, F). Scale bars, 50 μm. Tissue analyses were done in duplicate on seven (DBZ+38 h) or three (DBZ+72 h, DBZ+144 h) mice.
