Extended Data Figure 7: Generation chimaeras with STAP cells.

a, 2N chimaeras generated with STAP cells derived from Oct4-gfp C57BL/6 mice (left) and 129/Sv?×?C57BL/6 F₁ mice (right). b, Generation of chimaeric mice from STAP cells by cluster injection. STAP cells used in the experiments above were generated from CD45⁺ lymphocytes of multiple neonatal spleens (male and female tissues were mixed). *All fetuses were collected at 13.5?d.p.c. to 15.5?d.p.c. and the contribution rate of STAP cells into each organ was examined by FACS. **The contribution of STAP cells into each chimaera was scored as high (>50% of the coat colour of GFP expression). ***B6GFP: C57BL/6 mouse carrying cag-gfp.c, Production of offspring from STAP cells via germline transmission. Chimaeras generated with 129/Sv?×?B6GFP STAP cells (obtained from the experiments shown in b) were used for germline transmission study. d, 4N embryos at E9.5 generated with STAP cells derived from F₁ GFP mice (B6GFP and DBA/2 or 129/Sv). B6GFP, C57BL/6 mouse carrying cag-gfp.