Extended Data Figure 4: Teratoma formation assay and characterization of Oct4-GFP-dim cells.

a–c, Teratomas formed from STAP cell clusters included neuroepithelium (a), striated muscle (b) and pancreas (c; right, high-magnification view showing a typical acinar morphology and ductal structures). Scale bars, 100?μm. d, Teratoma-forming ability of Oct4-GFP⁺ and Oct4-GFP-dim cells (isolated by FACS, top). Oct4-GFP⁺ cells, but not Oct4-GFP-dim cells, efficiently formed teratomas (table at the bottom). However, because STAP cells were dissociation-intolerant, the teratoma-forming efficiency of dissociated Oct4-GFP⁺ cells was lower than that of non-dissociated STAP cell clusters. e, Gene expression of Oct4-GFP⁺ and Oct4-GFP-dim cells (n = 3, the average?±?s.d.). Haematopoietic marker gene expression (left) and early lineage marker gene expression (right) are shown.